SCIENTIFIC CATALOG
Diagnostic Enzyme
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24 items
phi29 DNA Polymerase
Phi29 DNA polymerase is a thermophilic DNA polymerase cloned from the B. subtilis phage phi29. It has high processivity and strand displacement activity, allowing it to continuously synthesize DNA fragments up to 70kb, making it great for efficient isothermal DNA amplification with high fidelity. There are two types of phi29 DNA polymerase available—one heat-resistant and one highly active—both of which can be used for nucleic acid and gene testing.
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Thermostable T7 RNA Polymerase
This product is a genetically engineered DNA-dependent RNA polymerase that is highly specific to the T7 phage promoter. Compared to wild-type T7 RNA polymerase, it can efficiently transcribe in vitro at temperatures between 37 and 54°C.
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Uricase
Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. Uricase can be used for the determination of uric acid in biological fluids.
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Proteinase K
Proteinase K catalyzes the hydrolysis of peptide bonds in various proteins, subsequently forming small peptide fragments or amino acids. Proteinase K can be used for nucleic acid extraction, protein removal, and sample lysis.
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Creatinase
Creatine kinase (CRE) can further catalyze the hydrolysis of creatine to produce creatinine and urea. CNH, CRE, and SOX together can be used to measure creatinine levels in biological samples like serum and urine, as well as for clinical biochemical tests related to kidney function.
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Lactate 2-monooxygenase
Lactate oxidase is a flavin mononucleotide (FMN)-dependent enzyme that catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide, with FMN as a cofactor. This enzyme facilitates the indirect determination of L‑lactate by measuring the hydrogen peroxide formed in the reaction.
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Glucose-6-Phosphate Dehydrogenase
Glucose-6-phosphate dehydrogenase can catalyze the oxidation of glucose-6-phosphate to 6-phosphoglucono-δ-lactone in the presence of NADP⁺, with NADP⁺ being reduced to NADPH at the same time. Glucose-6-phosphate dehydrogenase can be used for screening G6PD deficiency, assessing the risk of hemolytic anemia, testing red blood cell enzyme activity, and conducting clinical biochemical analysis.
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Creatinine amidohydrolase
Creatinine enzyme (CNH) can catalyze the hydrolysis of creatinine into creatine. CNH, CRE, and SOX together can be used to measure creatinine levels in biological samples like serum and urine, as well as for clinical biochemical tests related to kidney function.
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Chlesterol Esterase
Cholesterol esterase is an enzyme with broad substrate specificity, playing a key role in regulating the bile salt-mediated hydrolysis of dietary cholesterol esters, and it also participates in the hydrolysis of triglycerides and phospholipids. It's mainly used to measure total cholesterol levels in serum and plasma.
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L-Lactic dehydrogenase
Lactate dehydrogenase can reversibly catalyze the oxidation of L-lactate to pyruvate in the presence of NAD⁺, accompanied by the reduction of NAD⁺ to NADH and the release of H⁺. Lactate dehydrogenase can be used for checking heart enzyme profiles, evaluating liver function, determining sample hemolysis, and enzymatically measuring L-lactate content.
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HRP
Horseradish peroxidase (HRP) is isolated from horseradish (Armoracia rusticana) and belongs to the heme-containing peroxidases. It's used in biochemical applications like Western blot, ELISA, and immunohistochemistry. HRP helps amplify weak signals and makes target molecules like proteins easier to detect.
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Sarcosine oxidase
Creatine oxidase (SOX) can catalyze the oxidation of creatine to produce glycine, formaldehyde, and hydrogen peroxide; CNH, CRE, and SOX together can be used to measure creatinine levels in biological samples like serum and urine, as well as for clinical biochemical tests related to kidney function.
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